PROBLEM:
To examine the effect of interleukin-10 on production regulation production of the gelatinases by amniochorion in an in vitro model of infection.
METHODS:
We placed amniochorionic membranes collected from eight women who had elective repeat cesareans at term in an organ explant culture system. After 48 hours in culture, the membranes were stimulated with lipopolysaccharide (lipopolysaccharide-50 ng/ml) and some were co-stimulated with interleukin-10 (500 ng/ml). Tissue and media samples were collected after 24-hour stimulation. Quantitative polymerase chain reactions and enzyme-linked immunosorbent assays were used to evaluate matrix metalloproteinase 2 and matrix metalloproteinase 9 messenger RNA and proteins, respectively.
RESULTS:
Lipopolysaccharide stimulation induced 55.14 transcripts of matrix metalloproteinase 9, compared with 0.83 in control tissues (P = .0001) Co-stimulation with interleukin-10 and lipopolysaccharide significantly reduced matrix metalloproteinase 9 messenger RNA levels to 10 transcripts (P = .0001). Lipopolysaccharide stimulation produced 29.25 ng/ml of immunoreactive matrix metalloproteinase 9,which was reduced to 6.3 ng/ml (Padj= .016) after co-stimulation with interleukin-10. Although not significant matrix metalloproteinase 2 messenger RNA levels were higher in lipopolysaccharide stimulated tissues (4.37 x 106 transcripts) compared with control (2.8 x 105 transcripts; P = .08) with a significant decrease in matrix metalloproteinase 2 messenger RNA levels in interleukin-10 co-stimulated tissues (2.9 x 106; Padj = .007). Interleukin-10 co-stimulation resulted in a significant decrease in matrix metalloproteinase 2 protein production (203.1 [lipopolysaccharide] and 149.75 [with interleukin-10]; P = .0003).
CONCLUSIONS:
Interleukin -10 eliminated lipopolysaccharide induction of matrix metalloproteinase 2 and 9 in amniochorion.